The pups died in early September.
“EEE is very, very rare in canines,” zoo officials said. “The zoo animals that are susceptible to EEE (zebras, Przewalski’s horses and ostriches) are vaccinated annually against EEE but there is no approved vaccine for canines.”
Zoo officials said that the pups were part of a litter born to a breeding pair of Mexican gray wolves on June 14 at the zoo. The surviving wolf pup and both parents appear healthy and are being monitored closely by the veterinarian and animal care staff.
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